Composite

Part:BBa_K4345017

Designed by: Michelle Patricia   Group: iGEM22_KU_Leuven   (2022-09-30)
Revision as of 11:01, 30 September 2022 by Registry (Talk | contribs)

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pSynSense2.5000 PfdeAR-FdeR biosensor circuit

Biosensor circuit in pSynSense2.5000 developed by De Paepe et al. (2018)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 580
    Illegal EcoRI site found at 1058
    Illegal XbaI site found at 1088
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 580
    Illegal EcoRI site found at 1058
    Illegal NheI site found at 1523
    Illegal NotI site found at 1094
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 580
    Illegal EcoRI site found at 1058
    Illegal BamHI site found at 1073
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 580
    Illegal EcoRI site found at 1058
    Illegal XbaI site found at 1088
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 580
    Illegal EcoRI site found at 1058
    Illegal XbaI site found at 1088
    Illegal NgoMIV site found at 767
    Illegal NgoMIV site found at 779
    Illegal NgoMIV site found at 870
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1544


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