Composite

Part:BBa_K4274030

Designed by: Jiawen Chen   Group: iGEM22_KEYSTONE   (2022-09-30)
Revision as of 10:34, 30 September 2022 by Registry (Talk | contribs)

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ptac-RiboJ-B0034-ClSS_S533A-B0034-ERG20-B0015

The expression cassette ptac-RiboJ-B0034-ClSS_S533A-B0034-ERG20-B0015 includes ClSS_S533A (Part: BBa_K4274000) and ERG20 (Part: BBa_K849001). It is used to heterologously express santalene synthase and farnesyl pyrophosphate synthase in E. coli strain DH5a (tnaA-), which take part in the alpha-santalene production pathways of IPP ⇄DMAPP → GPP → FPP → alpha-santalene. This part is also composed of the IPTG-inducible promoter ptac-RiboJ, the ribosomal binding site B0034 (Part: BBa_B0034), and the strong terminator B0015 (Part: BBa_B0015).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1555
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None