Composite
Part:BBa_K4274030
Designed by: Jiawen Chen Group: iGEM22_KEYSTONE (2022-09-30)
ptac-RiboJ-B0034-ClSS_S533A-B0034-ERG20-B0015
The expression cassette ptac-RiboJ-B0034-ClSS_S533A-B0034-ERG20-B0015 includes ClSS_S533A (Part: BBa_K4274000) and ERG20 (Part: BBa_K849001). It is used to heterologously express santalene synthase and farnesyl pyrophosphate synthase in E. coli strain DH5a (tnaA-), which take part in the alpha-santalene production pathways of IPP ⇄DMAPP → GPP → FPP → alpha-santalene. This part is also composed of the IPTG-inducible promoter ptac-RiboJ, the ribosomal binding site B0034 (Part: BBa_B0034), and the strong terminator B0015 (Part: BBa_B0015).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1555
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |