Composite

Part:BBa_K4468008

Designed by: Zhichao Li   Group: iGEM22_HUST-China   (2022-09-30)
Revision as of 09:55, 30 September 2022 by Registry (Talk | contribs)

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T7-PmrA-T7-PmrB-T7 Terminator-PmrC-Oprf-Sitag-T7 Terminator

Description This is a composite component for the absorption and recovery of lanthanides, especially for Tb3+. It consists of T7-PmrA-T7-PmrB(dLBT)-T7 Terminator-PmrC-oprf-Sitag-T7 Terminator. Induced by IPTG, it can express PmrA and PmrB protein. Besides, lanthanide ions in the external solution can initiate the expression of oprf-Sitag and let our protein bind on silica column with its engineered bacteria together.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 749


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