![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K4468008
Designed by: Zhichao Li Group: iGEM22_HUST-China (2022-09-30)
T7-PmrA-T7-PmrB-T7 Terminator-PmrC-Oprf-Sitag-T7 Terminator
Description This is a composite component for the absorption and recovery of lanthanides, especially for Tb3+. It consists of T7-PmrA-T7-PmrB(dLBT)-T7 Terminator-PmrC-oprf-Sitag-T7 Terminator. Induced by IPTG, it can express PmrA and PmrB protein. Besides, lanthanide ions in the external solution can initiate the expression of oprf-Sitag and let our protein bind on silica column with its engineered bacteria together.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 749
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Categories
Parameters
None |