Coding

Part:BBa_K200008

Designed by: Charles Fracchia, Royah Vaezi, Tianyi Wang, Nuri Purswani   Group: iGEM09_Imperial College London   (2009-08-12)
Revision as of 21:10, 26 August 2009 by Dineka (Talk | contribs)

Phenylalanine hydroxylase


Phenylalanine hydroxylase (PAH) encodes the enzyme phenylalanine hydroxylase that is the rate-limiting step in phenylalanine catabolism. Deficiency of this enzyme activity results in the autosomal recessive disorder phenylketonuria.


As part of the Imperial 2009 iGEM E.ncapsulator project, E.coli was engineered to synthesise PAH to a tunable threshold, and successfully coat and protect it through the stomach, until release in the small intestine.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 287
    Illegal BamHI site found at 814
    Illegal XhoI site found at 524
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None