Coding
g3p

Part:BBa_K257001:Design

Designed by: Guillaume Cambray & Guillaume Beauclair   Group: iGEM09_Paris   (2009-06-20)
Revision as of 20:15, 26 August 2009 by Beauclair (Talk | contribs) (→‎References)

Gene 3 protein of filamentous phages without the 18 first amino acids


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 592
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The g3p sequenced comes from the phage lambda genome.

It's a protein involved in the adhesion of the phage to the bacteria.

Its sequence is surounded by the biobrick enzymatic restriction sites.

Source

bla

References

  • The Mechanism of Bacterial Infection by Filamentous Phages Involves Molecular Interactions between TolA and Phage Protein 3 Domains. Fredrik Karlsson, Carl A. K. Borrebaeck, Nina Nilsson, and Ann-Christin Malmborg-Hager
  • Interdomain interactions within the gene 3 protein of philamentous phage. Jean Chatellier, Oliver Hartley, Andrew D. Grifths, Alan R. Fershta, Greg Wintera, Lutz Riechmannb
  • A prokaryotic membrane anchor sequence: Carboxyl terminus of bacteriophage fl gene III protein retains it in the membrane. Jef D. Boeke AND Peter Model