Device

Part:BBa_K4408008

Designed by: Leyu Xu   Group: iGEM22_Worldshaper-NJBIOX   (2022-09-29)
Revision as of 08:24, 30 September 2022 by LucyShi 2018 (Talk | contribs)


Pthl-adhE2, Expression of adhE2 with Pthl promoter

This part is responsible for expressing adhE2 protein. The expression is controlled by a Pthl promoter with an ribosome binding site (RBS) and a Cpa fdx terminator. It consists of BBa_K3443002(Pthl), BBa_K4408001(RBS for adhE2), BBa_K1462060(adhE2), and BBa_K3585002(Cpa fdx terminator). In our program, we use this device to construct the synthesis pathways of butanol in Clostridium tyrobutyricum. By introducing adhE2，butyryl-CoA is converted to butyl aldehyde and further transformed to butanol. Pthl starts the transcription and Cpa fdx terminator ends the transcription.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None