Regulatory

Part:BBa_K4307004

Designed by: Chi Zhang   Group: iGEM22_Tsinghua   (2022-09-30)
Revision as of 07:36, 30 September 2022 by Registry (Talk | contribs)

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AttP & AttB

AttP and attB are two attachment sites of Bxb1 integrase, each of which is about 50 bp in length. Bxb1 integrase is capable of catalyzing site-specific recombination between two attachment sites (attP and attB) and inverting the DNA sequence flanked by these two opposing sites.

This time, we express Bxb1 integrase in bacteria and check its capability of induce inversion.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 24
    Illegal BsaI site found at 55
    Illegal BsaI.rc site found at 150


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