Composite

Part:BBa_K4192121

Designed by: Muhua Liu   Group: iGEM22_CAU_China   (2022-09-30)
Revision as of 06:34, 30 September 2022 by Registry (Talk | contribs)

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Plac-obcA-obcB-Plac-Fpoar, Oxalate secretion gene circuit

The expression products of obcA and obcB are enzymes needed to produce oxalate from citric acid and acetyl-CoA. And FpOAR protein is the oxalate efflux channel. The composite part of these three genes can efflux oxalic acid out of the thallus. We have already tested it in Pseudomonas fluorescens.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2735
    Illegal PstI site found at 397
    Illegal PstI site found at 2878
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2735
    Illegal PstI site found at 397
    Illegal PstI site found at 2878
    Illegal NotI site found at 2330
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2735
    Illegal BglII site found at 1541
    Illegal BglII site found at 3738
    Illegal XhoI site found at 304
    Illegal XhoI site found at 1428
    Illegal XhoI site found at 3550
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2735
    Illegal PstI site found at 397
    Illegal PstI site found at 2878
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2735
    Illegal PstI site found at 397
    Illegal PstI site found at 2878
    Illegal NgoMIV site found at 389
    Illegal NgoMIV site found at 720
    Illegal NgoMIV site found at 748
    Illegal NgoMIV site found at 784
    Illegal NgoMIV site found at 1014
    Illegal NgoMIV site found at 1791
    Illegal NgoMIV site found at 2208
    Illegal AgeI site found at 326
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//awards/composite_part/nominee
Parameters
None