![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K4192121
Designed by: Muhua Liu Group: iGEM22_CAU_China (2022-09-30)
Plac-obcA-obcB-Plac-Fpoar, Oxalate secretion gene circuit
The expression products of obcA and obcB are enzymes needed to produce oxalate from citric acid and acetyl-CoA. And FpOAR protein is the oxalate efflux channel. The composite part of these three genes can efflux oxalic acid out of the thallus. We have already tested it in Pseudomonas fluorescens.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2735
Illegal PstI site found at 397
Illegal PstI site found at 2878 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2735
Illegal PstI site found at 397
Illegal PstI site found at 2878
Illegal NotI site found at 2330 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2735
Illegal BglII site found at 1541
Illegal BglII site found at 3738
Illegal XhoI site found at 304
Illegal XhoI site found at 1428
Illegal XhoI site found at 3550 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2735
Illegal PstI site found at 397
Illegal PstI site found at 2878 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2735
Illegal PstI site found at 397
Illegal PstI site found at 2878
Illegal NgoMIV site found at 389
Illegal NgoMIV site found at 720
Illegal NgoMIV site found at 748
Illegal NgoMIV site found at 784
Illegal NgoMIV site found at 1014
Illegal NgoMIV site found at 1791
Illegal NgoMIV site found at 2208
Illegal AgeI site found at 326 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//awards/composite_part/nominee
None |