Coding

Part:BBa_K190027:Design

Designed by: Wilfred Poppinga   Group: iGEM09_Groningen   (2009-08-26)
Revision as of 10:26, 26 August 2009 by Registry (Talk | contribs)

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MBP-ArsR fusion protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 360
    Illegal BglII site found at 1271
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 58
    Illegal SapI.rc site found at 1102


Design Notes

Adding tobacco etch virus (TEV) cleavage site and linker site to facilitate cloning. Adding a SacI restriction site to facilitate non-expensive cloning.


Source

ArsR was obtained from the E. coli K-12 DH10B genome MBP was obtained from a plasmid

References