Coding

Part:BBa_K4220008

Designed by: Xiaoya Liu   Group: iGEM22_NEU_CHINA   (2022-09-25)
Revision as of 01:31, 30 September 2022 by Flora778 (Talk | contribs) (→‎Introduction)


P2Y2-P2A-mCherry

Introduction

The product coded by this sequence is used to show the position of P2Y2 and the function of P2A by mCherry reporter.

P2Y2

In order to increase its sensitivity and maximum response to sense eATP (extracellular ATP), we mutated K240N and F307S.[1]

P2A

After adding two proteins in the two sides of P2A, it will cleave by itself and release the two proteins at the same time.

mCherry

To report the successful cleavage of P2A peptide.

Results

1.The P2A peptide can work effectively:

caption

2.The production of P2Y2:

caption

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1159
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1095
    Illegal AgeI site found at 389
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 570
    Illegal SapI site found at 615


[edit]
Categories
Parameters
None