Part:BBa_K4194019

fadD

The gene codes for long chain acyl-CoA synthase(CoA ligase).

CoA ligase

CoA ligase catalyze the reaction of hydroxyl fatty acids with CoA

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 195
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 504
  Illegal XhoI site found at 1006
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 408
  Illegal AgeI site found at 1033
  
• 1000
  COMPATIBLE WITH RFC[1000]

Gas chromatographic analysis of hydrolyzed products

The BUCT-China team attempted to synthesize PHFA (polyhydroxyfatty acid, a biocompatible polymer material) using an enzyme system containing CoA Ligase.The obtained product was hydrolyzed and then analyzed by gas chromatography, and the result analysis showed that the monomer of the polymer was hydroxy hexadecanoic acid. Therefore, it can be seen that the system carried out hydroxylation reaction and subsequent polymerization reaction on the fatty acid of the substrate 16C as expected, and the system basically met our requirements.

Results of gas chromatographic analysis of product hydrolysis.

NMR hydrogen spectrogram analysis of polymers

The above fig. shows the NMR of the simulated dimer product and the below fig. shows the NMR of the product.

The characteristic peaks determines that the product has dimerization products.

NMR of hydroxyhexadecanoic acid.

NMR of dimeric compounds.