RNA

Part:BBa_K228001

Designed by: Lin Min   Group: iGEM09_PKU_Beijing   (2009-08-25)
Revision as of 08:49, 25 August 2009 by Registry (Talk | contribs)

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SupD-tRNA

The SupD-tRNA part functions as a tRNA, it suppresses the TAG amber stop codon and translate it into a Ser. There is no need to assembly SupD-tRNA with a rbs, since it is not translated.

This part, together with the T7ptag part, make an AND Gate, that is, in the presence of both the 2 parts, T7 polymerase is tranlated into a functional polymerase that transcrips a downstream element constructed after a T7 promoter. Which is the output of the AND Gate.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 33
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 36


[edit]
Categories
Parameters
n/aSupD-tRNA