Device

Part:BBa_K4375019

Designed by: Zska Csorba   Group: iGEM22_ELTE   (2022-09-29)
Revision as of 22:18, 29 September 2022 by Registry (Talk | contribs)

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Blue light inducible production of Cytolysin A (BLADE)

This device is meant for the light-inducible production of Cytolysin A (BBa_K811000), a pore-forming toxin. To achieve this, we used a modified arabinose operator system, where the transcription factor, AraC, is engineered to homodimerise by blue-light instead of arabinose binding. Its called BLADE and described in BBa_K4375003, and it is produced under the strong constitutive promoter J23101*.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 29
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None