Device

Part:BBa_K4375017

Designed by: Zska Csorba   Group: iGEM22_ELTE   (2022-09-29)
Revision as of 20:59, 29 September 2022 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


anti-CEA Nanobody Display System

This construct is meant for detecting cancer cells that express CEA, carcioembrional antigen by bacterial cells. This device contains two different constructs whose production is under the control of a constitutive strong promoter J23101* (BBa_K4375003). CEA-positive cell-specific attachment is achieved by an anti-CEA-specific Nanobody, (BBa_K4375006 ). The Nanobody displayed on the bacteria outer membrane, by the N-terminal domain of a bacterial adhesion factor, Intimin, described in BBa_K4375012. Cancer cell specific interaction can be detected by two epitope tags: Myc (BBa_K4375014) and anti-E, or via the fluorercence of the policistronicly expressed sfGFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 29
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2043
    Illegal BamHI site found at 4059
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2064
    Illegal NgoMIV site found at 4080
    Illegal AgeI site found at 4146
    Illegal AgeI site found at 4278
    Illegal AgeI site found at 4678
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None