Part:BBa_K4447004:Design

EryK coding sequence

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 1197
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

A gly-gly-ser spacer, and a polyhistidine-tag were added before stop codon for protein purification. NcoI and XhoI restriction sites were added in 5' and 3' ends for protein overexpression in pBAD/Myc-His plasmids. There are no scars between each protein.

Source

The original sequence was reported by Stassi et al. (1993). Sequence can be obtained through GenBank (L05776). The sequence for BBa_K1159302 was reported by Poëa-Guyon </i>et al.</i> (2013). The sequence for BBa_K1907000 was reported by Nagai et al. (2002).

References

[1]. Nagai, T., Ibata, K., Park, E. S., Kubota, M., Mikoshiba, K., & Miyawaki, A. (2002). A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nature Biotechnology, 20(1), 87–90. doi:10.1038/nbt0102-87

[2]. Poëa-Guyon, S., Pasquier, H., Mérola, F., Morel, N., & Erard, M. (2013). The enhanced cyan fluorescent protein: a sensitive pH sensor for fluorescence lifetime imaging. Analytical and bioanalytical chemistry, 405(12), 3983–3987. https://doi.org/10.1007/s00216-013-6860-y

[3]. Stassi, D., Donadio, S., Staver, M. J., & Katz, L. (1993). Identification of a Saccharopolyspora erythraea gene required for the final hydroxylation step in erythromycin biosynthesis. Journal of bacteriology, 175(1), 182–189. https://doi.org/10.1128/jb.175.1.182-189.1993