Composite

Part:BBa_K4192113

Designed by: Lehan Dong   Group: iGEM22_CAU_China   (2022-09-29)
Revision as of 18:07, 29 September 2022 by Registry (Talk | contribs)

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Plac-RBS-cdg

This part is used to test the role of cdg (BBa_K4192010) in biofilm production. We used IPTG to induce its expression, and then used crystal violet to dye 96 well plates. The results are shown in the figure below.

The effect of this part is not significant，which means it is hard to work alone. However, it shows obvious enhancement effect when it interacts with gacA gene (BBa_K4192011).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 468
    Illegal BamHI site found at 459
    Illegal XhoI site found at 557
    Illegal XhoI site found at 1001
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 392
    Illegal AgeI site found at 959
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 410
    Illegal BsaI.rc site found at 977


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Categories
Parameters
None