Composite

Part:BBa_K4192110

Designed by: Lehan Dong   Group: iGEM22_CAU_China   (2022-09-29)
Revision as of 17:14, 29 September 2022 by OwenDong (Talk | contribs)


cdg-gacA

RBS-cdg-RBS-gacA is composed of cdg gene (BBa_K4192010), gacA gene (BBa_4192011) and strong RBS sequence of Pseudomonas fluorescens (BBa_K4192000). cdg and gacA encode regulatory factors DGC and GacA in Pseudomonas fluorescens, which can ncrease the expression of extracellular polysaccharide (EPS) and then increase the production of biofilm. This part has been tested to be effective in Pseudomonas fluorescens 2P24.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 268
    Illegal BamHI site found at 259
    Illegal XhoI site found at 357
    Illegal XhoI site found at 801
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 192
    Illegal NgoMIV site found at 1289
    Illegal AgeI site found at 759
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 210
    Illegal BsaI.rc site found at 777


[edit]
Categories
Parameters
None