![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K4212008:Design
Designed by: Shirin Bamezai Group: iGEM22_Imperial_College_London (2022-09-29)
GerA_operon
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 3506
Illegal PstI site found at 521
Illegal PstI site found at 3457 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 3506
Illegal PstI site found at 521
Illegal PstI site found at 3457 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 3506
Illegal BglII site found at 3038
Illegal XhoI site found at 628 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 3506
Illegal PstI site found at 521
Illegal PstI site found at 3457 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 3506
Illegal PstI site found at 521
Illegal PstI site found at 3457 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 2693
Design Notes
Codon optimised to remove recognition sites for BbsI, BsaI and BsmbI to enable assembly in Golden Gate system.
Source
Native sequence from B. subtilis genome.