Coding

Part:BBa_K4212005

Designed by: Shirin Bamezai   Group: iGEM22_Imperial_College_London   (2022-09-29)
Revision as of 15:47, 29 September 2022 by Registry (Talk | contribs)

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gRNA_SDP

Designed for CRISPR system introducing double stranded cut in origin of replication of BBa_K4212031 part (Golden Gate level 2 B. subtilis expression vector). Useful for production of self-digesting plasmid system functional in B. subtilis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None