Part:BBa_K4212004
CotZ_ChiS
This part codes for a fusion protein consisting of the B. subtilis native CotZ anchor protein and a B. pumilus chitinase enzyme. The CotZ protein localizes in the outer coat of B. subtilis spores, enabling spore display and greater accessibility to . In this case with the chitinase fusion, spores will display chitinases on the surface capable of breaking down chitin into its monomer components, such as N-acetylglucosamine. In the context of our project, the chitinase activity enables us to equip spores with the ability to breakdown the chitin-rich outer coat of fungal pathogens, not only inhibiting their activity but generating chitin monomers that can function as a biomarker, as well as an inducer of plant systemic immune response. This fusion includes a rigid linker, to reduce steric hindrance between the two protein domains and enable greater chitinase activity. The same was selected for testing following dry lab modelling.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 705
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 705
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 705
Illegal BglII site found at 48
Illegal BamHI site found at 690 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 705
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 705
- 1000COMPATIBLE WITH RFC[1000]
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