Part:BBa_K4469004
hTERT-CMV (TMC) fusion promoter
The hTERT-CMV (hTC or TMC) fusion promoter is a promoter that combines a fragment of hTERT promoter, which is from bases -456 to -2 upstream of the start codon, and a minimized fragment of CMV promoter, which is of bases -1017 to -901 upstream of the human CMV major immediate early protein start codon (Davis et. al, 2006). The promoter was first described in an oncolytic virotherapy that inserts the promoter in front of a GFP signal and E1A gene which is necessary for transcription of various proteins in adenoviral replication and thus leading to cell death(ibid). It is shown that the efficiency between a CMV promoter and a hTC promoter is comparable such that only the adenovirus with CMV or hTC promoter undergoes effective viral replication and gives visible GFP light in cancer cell lines (fresh DLD-1 cells)(ibid). In terms of cancer specificity of hTC promoter, the E1A expression was only detected in lung cancer cell line H1299, pancreatic cancer cell line MiaPaca-2 and colon cancer cell line DLD-1, but not in infected normal cell line NHFBs in the experiment(ibid). The promoters in the same plasmid design have also been tested in tumor-bearing mice whereas the results show that the viral transgene expression and replication were efficient in tumour, proven by detectable GFP signals in the tumor(ibid). The promoter was further proven in another literature as a promoter for delivering truncated CD19 to cancer surface as a tumor tag (Tang et. al, 2020). It is shown that while compared to hTERT and Survivin-driven promoters, the truncated CD19 were even better expressed by using TMC promoters(ibid).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 458
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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