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Part:BBa_K4212000
CotG_ChiS
This part codes for a fusion protein consisting of the B. subtilis native CotG anchor protein and a B. pumilus chitinase enzyme. The CotG protein has a midspore localization and high abundance in B. subtilis spores, enabling spore display. In this case with the chitinase fusion, spores will display chitinases on the surface capable of breaking down chitin into its monomer components, such as N-acetylglucosamine. In the context of our project, the chitinase activity enables us to equip spores with the ability to breakdown the chitin-rich outer coat of fungal pathogens, not only inhibiting their activity but generating chitin monomers that can function as a biomarker, as well as an inducer of plant systemic immune response.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 849
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 849
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 849
Illegal BamHI site found at 834 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 849
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 849
- 1000COMPATIBLE WITH RFC[1000]
None |