Coding
Part:BBa_K4192011:Design
Designed by: Lehan Dong Group: iGEM22_CAU_China (2022-09-29)
gacA from Pseudomonas fluorescens str. 2P24
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 316
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The effect of this gene is not stable, and increasing its expression can effectively improve the production of biofilm. However, there is a large difference between the parallel controls. It can play a better role when co-expressed with other regulatory factors such as cdg (BBa_K4192010).
Source
It comes from the genome of Pseudomonas fluorescens 2P24.