Coding

Part:BBa_K4192010

Designed by: Lehan Dong   Group: iGEM22_CAU_China   (2022-09-29)
Revision as of 14:31, 29 September 2022 by Alexandra L (Talk | contribs)


cdg gene from Pseudomonas fluorescens str.2P24

The cdg (BBa_K4192010) encodes the DGC protein of Pseudomonas fluorescens,also named as dgc. DGC protein catalyzes the synthesis of r c-di-GMP,which is an important regulator[1]. It can promote bacterial adhesion, extracellular polysaccharide synthesis and other functions. Increasing its expression can increase the production of biofilm.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 263
    Illegal BamHI site found at 254
    Illegal XhoI site found at 352
    Illegal XhoI site found at 796
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 187
    Illegal AgeI site found at 754
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 205
    Illegal BsaI.rc site found at 772



References

[1]Ivanov, Ivan E et al. “Atomic force and super-resolution microscopy support a role for LapA as a cell-surface biofilm adhesin of Pseudomonas fluorescens.” Research in microbiology vol. 163,9-10 (2012): 685-91. doi:10.1016/j.resmic.2012.10.001


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