Translational_Unit

Part:BBa_K190021:Design

Designed by: Nienke Kuipers   Group: iGEM09_Groningen   (2009-08-19)
Revision as of 15:43, 19 August 2009 by Nienke (Talk | contribs) (→‎References)

SmtA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 111
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 111
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 111
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 111
    Illegal AgeI site found at 139
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The BioBrick prefix and suffix were added by PCR, also the RBS (B0034) was added.


Source

The gene was cloned from pET29a-SmtA (kindly provided by Kevin Waldron, Newcastle University), it's origin is from the genome of Synechococcus PC

References

  • Microbial Metallothioneins, Robinson et al. Adv in MicroPhys, 2001, 44; 183-213
  • Cyanobacterial metallothionein gene expressed in Escherichia Metal-binding properties of the expressed protein, Jianguo Shi et al. FEBS letters, June 1992, Volume 303, number 2,3, 159-163.