Translational_Unit

Part:BBa_K190019:Design

Designed by: Nienke Kuipers   Group: iGEM09_Groningen   (2009-08-19)
Revision as of 14:32, 19 August 2009 by Nienke (Talk | contribs) (→‎Design Notes)

fMT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 38
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 127
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Add BioBrick prefix and suffix, also a RBS (B0034) will be added (not in sequence yet)

Source

fMT was cloned from pMAL-MT (kindly provided by Wilfred Chen, University of California), the origin of the gene is the genome of Fucus vesiculosus.

References

Highly Selective and Rapid Arsenic Removal by Metabolically Engineered Escherichia coli Cells Expressing Fucus vesiculosus Metallothionein. Singh et al. App and Env Microbiology, May 2008, p. 2924–2927