Composite

Part:BBa_K4408005:Design

Designed by: Leyu Xu   Group: iGEM22_Worldshaper-NJBIOX   (2022-09-29)
Revision as of 10:07, 29 September 2022 by Registry (Talk | contribs)

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thl modified by oligopeptide SpyCatcher


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1076
    Illegal SapI.rc site found at 900


Design Notes

It can be bounded with SpyTag-linker 1-hbd (BBa_K4408006) to enhance the production of butanol. Thl and hbd are two key enzymes in the metabolic pathway of chlostridium butyricum to transform Acetyl-CoA to 3-Hydroxybutytyl-CoA. By linking these two enzymes together, we can improve the utilization of reagants and the reaction rate of the transformation, and thus improve the production of butanol and butyl acid.


Source

Linker 1 is based on part BBa_K4408003 thl is based on part BBa_K3443003 SpyCatcher is based on part BBa_K3329016

References