Part:BBa_K4140015

Kink turn

Part Description

A common structural motif identified in functioning RNA species is the kink-turn (k-turn). It typically consists of a tandem trans sugar edge-Hoogsteen G followed by a three-nucleotide bulge: pair A bases. The minor grooves are juxtaposed and the axis of duplex RNA is given a strong bend. The conserved adenine nucleobases of the G:A basepairs accept the cross-strand H-bonds that form at the interface. The k-turns are split into two conformational classes, N3 and N1, by alternative acceptors for one of these. The conformation that a specific k-turn adopts is determined by the base pair (3b:3n) that follows the G:A pairings. K-turns typically bind proteins and mediate tertiary contacts in folded RNA species. Members of the L7Ae family of proteins are frequently found to bind k-turns

Usage

using K-turns' capacity to mediate tertiary contacts in folded RNA species and bind proteins. As a result, we employ it to regulate the expression of our CRISPR regulatory system since, when combined with L7Ae, it suppresses the translation of the protein portion (Cas12g),  Whenever there is a high quantity of phenylalanine.  

Literature Characterization

Figure 1.Substrate RNA cleavage assay using wild-type and mutant target RNAs.

References

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]