RNA

Part:BBa_K3743019

Designed by: Ahmed Gamal Mohamed Mattar   Group: iGEM21_AFCM-Egypt   (2022-09-24)
Revision as of 09:52, 29 September 2022 by Mohamed-Ab0elghare (Talk | contribs) (→‎Usage)

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gRNA Binding site


Part Description

Complementary part to crRNA present down stream to the target gene

Usage

we employed this part to attract our regulatory system Cas12g guided by specific RNA to PAH mRNA that we want to stop its translation in case of overexpression of PAH or high levels OF tyrosine.

Literature Characterization

Figure 1.Substrate RNA cleavage assay using wild-type and mutant target RNAs.





























References

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None