Part:BBa_K4365000
FIG1 inducible promoter
The Factor-Induced Gene 1 (FIG1) is a pheromone-induced promoter in yeast that is activated by the alpha mating factor. The FIG1 promoter is a great device for synthetic biology applications aiming to engineer productive stationary-phase systems in S. cerevisiae. This is because the induction of the FIG1 promoter by the alpha mating factor, in addition to activating expression, leads to the arrest of growth and maintenance of active metabolism in S. cerevisiae. As a result, the synthesis of a product of interest is decoupled from population growth, and cellular resources, such as carbon and nitrogen, can be redirected from biomass production to the synthesis of the desired bioproduct. Moreover, the FIG1 promoter is strictly regulated by a well-understood signaling cascade, which avoids cross-activation of other pathways and has enabled the construction and fine-tuning of a multitude of synthetic regulatory circuits.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 172
- 1000COMPATIBLE WITH RFC[1000]
//promoter
//regulation/positive
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