Coding
Lpp

Part:BBa_K4385001:Design

Designed by: Min An Zhao   Group: iGEM22_Jilin_China   (2022-09-29)
Revision as of 08:54, 29 September 2022 by Registry (Talk | contribs)

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Lipoprotein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PcusC is amplified from E. coli genomic DNA. The promoter can receive the upstream signal from the CusSR two-component membrane associated sensor kinase system, and inducibly express the downstream target gene. The expression intensity of PcusC has a certain positive correlation with the received signal intensity. By adjusting the external copper ion concentration, the expression intensity of PcusC can be adjusted.


Source

E.coli BL21 genomic sequence

References