Regulatory

Part:BBa_K4140006

Designed by: Ahmed Gamal Mohamed Mattar   Group: iGEM22_AFCM-Egypt   (2022-09-24)
Revision as of 08:29, 29 September 2022 by Mohamed-Ab0elghare (Talk | contribs) (→‎Usage)


TyrP promoter


Part Description

TyrP Promoter is an inducible promoter found in E.coli chormosomes and more specifically K-12 chromosome. This promoter works and is induced when both tyrR gene product & Phenyalanine are present causing increased tyrP transcription.

Usage

TyrP promoter,normally regulate the expression for a part which codes for tyrosine-specific transport system, it is induced when phenylalanine and the tyrR gene product (TyrR dimer)are together ,taking advantage of that we use it to regulate the expression of PAH in our therapeutic circuit and ß-galactosidase in our diagnostic circuit.

Literature Characterization

Figure 1.Substrate RNA cleavage assay using wild-type and mutant target RNAs.





























References

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None