Part:BBa_K4140005

L7AE

Part Description

A protein called L7Ae that binds to RNA inhibits the targeted transcript's ability to be translated. Transcriptional repressors take longer to suppress the intended construct than L7Ae that regulate gene expression at the translational level. As it specifically targets a sequence on the 5' end of the RNA termed the 2x-kturn. L7Ae can be used to build more complicated genetic circuits that are regulated at both the translational and transcriptional levels

Usage

L7Ae that binds to RNA inhibits the targeted transcript's ability to be translated. As it specifically targets a sequence on the 5' end of the RNA termed the 2x-kturn. as a post transcriptional modifier we use it to repress the translation of cas12g protein in case of high levels of phenylalanine and TyrR that leads to inhibiting CRISPR system activity and preserving the expression of PAH.

Literature Characterization

Figure 1.Substrate RNA cleavage assay using wild-type and mutant target RNAs.

References

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]