Part:BBa_K4475000

CTB1 Gene from Cercospora beticola

This is the full exon only (cDNA) coding sequence for the CTB1 enzyme in the fungus cercospora beticola. The enzyme is the first in a multi step pathway for the production of cercosporin, a reactive oxygen species producing toxin in the presence of sun light. FSU's iGEM team is looking to synthesize CTB1 in yeast as a proof of concept for cercosporin biosynthesis in a non-native and scalable organism, with the goal of producing cercosporin to combat algal blooms. The coding sequence has been codon optimized for Saccharomyces cerevisiae and modified to remove some illegal iGEM assembly cut sites via silent mutations. An HA tag is embedded on the N-terminus for expression verification.

Source https://www.ncbi.nlm.nih.gov/nuccore/1331157726

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 3532
  Illegal BamHI site found at 3441
  Illegal BamHI site found at 5614
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 6121
  Illegal AgeI site found at 4195
  
• 1000
  COMPATIBLE WITH RFC[1000]