Coding

Part:BBa_K4194019

Designed by: Jiong Huang   Group: iGEM22_BUCT-China   (2022-09-26)
Revision as of 04:19, 29 September 2022 by HJ (Talk | contribs)


fadD

The gene codes for long chain acyl-CoA synthase(CoA ligase).


Usage and Biology

CoA ligase

CoA ligase catalyze the reaction of hydroxyl fatty acids with CoA



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 195
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 504
    Illegal XhoI site found at 1006
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 408
    Illegal AgeI site found at 1033
  • 1000
    COMPATIBLE WITH RFC[1000]

Experiment and result

The BUCT-China team attempted to synthesize PHFA (polyhydroxyfatty acid, a biocompatible polymer material) using an enzyme system containing CoA Ligase.The obtained product was hydrolyzed and then analyzed by gas chromatography, and the result analysis showed that the monomer of the polymer was hydroxy hexadecanoic acid. Therefore, it can be seen that the system carried out hydroxylation reaction and subsequent polymerization reaction on the fatty acid of the substrate 16C as expected, and the system basically met our requirements.

Results of gas chromatographic analysis of product hydrolysis.
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Categories
Parameters
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