DNA

Part:BBa_K4130014

Designed by: Sarah Broas   Group: iGEM22_Rochester   (2022-09-28)
Revision as of 03:42, 29 September 2022 by Registry (Talk | contribs)

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Aptamer for free Sarcosine

This part selectively detects sarcosine with a detection range of 5pM-50uM

Aptamers are oligonucleotides (ssDNA/ssRNA molecules) that have selective binding capabilities to target molecules such as proteins or small molecules. Binding events occur when the target molecule makes contact with the active site of the aptamer, causing the aptamer to fold. This

The first paper developed an aptamer in urine-like buffer conditions to maintain a dissociation constant of 0.33nM, indicating strong binding


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 67
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 67
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 67
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 67
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None