Regulatory

Part:BBa_K4387000

Designed by: Jana Mehdy, Lea Bruellmann, Marine Mausy   Group: iGEM22_UZurich   (2022-09-28)
Revision as of 19:43, 28 September 2022 by Jmehdy (Talk | contribs)


Nitric Oxide Sensing Promoter pNorVβ

PnorV_beta is an optimized nitric oxide sensitive promoter. The promoter is coupled to a positive feedback loop with sfGFP and NorR, the corresponding transcription factor. IHF was removed from the promoter PnorV to exhibit good sensitivity and dosage response at a low range of inducer DETA/NO, the used NO source in the experiment, activating the downstream genes' transcription.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//awards/basic_part/winner
Parameters
None