Regulatory
Part:BBa_K4387000:Design
Designed by: Jana Mehdy, Lea Bruellmann, Marine Mausy Group: iGEM22_UZurich (2022-09-28)
Nitric Oxide Sensing Promoter pNorVβ
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
No codon optimization was necessary. The sequence was taken directly from the reference paper.
Source
The sequence of the PnorV_beta was obtained from the paper of Xiaoyu J. Chen et al. [1]
References
[1]Xiaoyu J. Chen, Baojun Wang, Ian P. Thompson, and Wei E. Huang et al. Rational Design and Characterization of Nitric Oxide Biosensors in E. coli Nissle 1917 and Mini SimCells ACS Synthetic Biology 2021 10 (10), 2566-2578 DOI: 10.1021/acssynbio.1c00223