Part:BBa_K3743020

VSVg-Fusogen

Part Description

A guide RNA (gRNA) is an RNA that is responsible for acting as a guide for enzymes that target DNA or RNA. Guide RNA targets their sequences by simple complementary base pairing of the target specific regions and it is used more specifically in applications that require targeted editinل of DNA such as found and present in the CRISPR-Cas9 or Cas 12 system.

Usage

It is the RNA domain of the CRISPR system which formed of two component crRNA that bind to the target sequence and tracrRNA that connect cas protein to the RNA which act as a handle we use this part to guide Cas12g protein to the complimentary site on mRNA of PAH to inhibit it’s expression when phenylalanine level is normal or to prevent over expression of PAH.

Literature Characterization

Figure 1.Substrate RNA cleavage assay using wild-type and mutant target RNAs.

References

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1396
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 1176
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 987
  
• 1000
  COMPATIBLE WITH RFC[1000]