Coding

Part:BBa_K4152012

Designed by: Junteng Wu   Group: iGEM22_TJUSLS_China   (2022-09-28)
Revision as of 18:37, 28 September 2022 by Registry (Talk | contribs)

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PK_MT12

To improve the performance of Proteinase K, we designed many Proteinase K mutant genes. PK_MT12 is a complicated mutation of Proteinase K, which contains 4 mutation sites: T16C-N257C-P175F-S197W.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 235
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 235
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 235
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None