Coding

Part:BBa_K4375012

Designed by: Zska Csorba   Group: iGEM22_ELTE   (2022-09-28)
Revision as of 18:30, 28 September 2022 by Registry (Talk | contribs)

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Intimin domain for surface display (with anti-E epitope tag)

This protein contains the N-terminal domain of intimin, which is a bacterial adhesion factor, D0 an ig-like protein, and an E epitope tag. Intimin beta-barrel transmembrane domain anchors the construct into the outer membrane of the bacteria.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1983
    Illegal BamHI site found at 3999
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2004
    Illegal NgoMIV site found at 4020
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None