RNA

Part:BBa_K3743019

Designed by: Ahmed Gamal Mohamed Mattar   Group: iGEM21_AFCM-Egypt   (2022-09-24)
Revision as of 17:59, 28 September 2022 by Ahmed Mattar (Talk | contribs) (→‎Usage)


glpD


Part Description

Complementary part to crRNA present down stream to the target gene

Usage

Complementary part to crRNA presents downstream to the target gene (PAH) its used to attract Cas12g to PAH mRNA that we want to stop its translation in case of overexpression of PAH or high levels tyrosine.

Literature Characterization

Figure 1.Substrate RNA cleavage assay using wild-type and mutant target RNAs.





























References

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 742
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 742
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 12
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 742
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 742
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None