Coding

Part:BBa_K4140010

Designed by: Ahmed Gamal Mohamed Mattar   Group: iGEM22_AFCM-Egypt   (2022-09-24)
Revision as of 17:51, 28 September 2022 by Ahmed Mattar (Talk | contribs) (→‎Usage)


Permease


Part Description

The bacterium Escherichia coli naturally transports phenylalanine across membranes via PheP (Phenylalanine-specific permease). PheP is a single, integral membrane transporter that uses the proton motive force to antiport L-phenylalanine and L-tyrosine. This transporter's activity under natural expression is known to range between 9 and 17,5.

Usage

(Phenylalanine-specific permease)naturally transports phenylalanine across membranes via PheP . integral membrane transporter that uses the proton motive force to antiport L-phenylalanine and L-tyrosine.we use the part to improve the permeability of cells to phenylalanine increasing its concentration intracellularly inducing our circuit to express PAH.

Literature Characterization

Figure 1.Substrate RNA cleavage assay using wild-type and mutant target RNAs.





























References

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 37
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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