Coding

Part:BBa_K4152105

Designed by: Qinting Lin   Group: iGEM22_TJUSLS_China   (2022-09-28)
Revision as of 17:44, 28 September 2022 by Registry (Talk | contribs)

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5

This composite part is made up with 6 basic parts: AOX1 promoter, α-factor secretion signal, Propeptide, our target protein WT, the His-tag, and AOX1 terminator. These elements make our target protein better expressed and more convenient for subsequent protein detection.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1504
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1504
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1725
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1504
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1504
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None