Translational_Unit
tphB

Part:BBa_K4378000:Design

Designed by: Leander Maximilian Linke   Group: iGEM22_Uppsala   (2022-09-28)
Revision as of 13:25, 28 September 2022 by Registry (Talk | contribs)

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TphB: An effective overexpression casette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 288
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 409


Design Notes

For potential in vitro use of the enzyme an T7-Tag is attached to allow purification through affinity chromatography.


Source

https://www.uniprot.org/uniprotkb/Q3C1E1/entry

References