![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K4122025
Designed by: Aiqi Luo Group: iGEM22_IvyMaker-China (2022-09-28)
+ SP-MHETase-cMyc-SNC-T-PGK1
+ SP-CBM-V5 -ST-ST-SNT-ST-7813Compose of 3 Parts:
P-SP-PETase-His-Spycatcher-T-ADH2
P-SP-MHETase-cMyc-Snoopcatcher-T-PGK1
SP-CBM-V5-ST-ST-SNT-ST-7813
In this assembly, SP is signal peptide, the same as last year (BBa_K4122014). Different from BBa_K4122020, we exchanged the position of tags and catchers.Besides, we also moved V5 tag before the spytag to fold correctly.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |