Tag
HlyA-tag

Part:BBa_K4387997:Design

Designed by: Nathalie Weibel   Group: iGEM22_UZurich   (2022-09-28)
Revision as of 12:45, 28 September 2022 by Registry (Talk | contribs)

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HlyA-tag obtained from the hemolysin A one-step secretion system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 565
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Shortening this sequence to the essential last 60 amino acids has not been tested yet.


Source

Sequence of the HlyD was obtain from the paper of Ruano-Gallego et al. [1]

References