Tag
HlyA-tag
Part:BBa_K4387997:Design
Designed by: Nathalie Weibel Group: iGEM22_UZurich (2022-09-28)
HlyA-tag obtained from the hemolysin A one-step secretion system
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 565
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Shortening this sequence to the essential last 60 amino acids has not been tested yet.
Source
Sequence of the HlyD was obtain from the paper of Ruano-Gallego et al. [1]