Coding

Part:BBa_K4247020:Design

Designed by: Akila Ravikumar   Group: iGEM22_UCopenhagen   (2022-09-28)
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Mfp151


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

MFP151 is a long protein and as such, its sequence might be difficult to synthesise. Hence, at the UCopenhagen team, we have split the sequence into two halves such that each half of the sequence would be produced in 2 expression plasmids each. These two halves can be put together by Golden Gate Cloning to obtain the sequence coding for the full MFP151 protein.

Both halves of the sequence coding for MFP151 are contained in an pET24 (+) expression vector having a T7 promoter, terminator, KAN resistance gene and a 6x His-tag in the C-terminus of the protein.

Further, this sequence was codon optimised as per E.coli's codon bias.



Source

The sequence of this composite part is obtained from the following basic parts: BBa_K4247018 (mfp151_first-half) and BBa_K4247019 (mfp151_second-half).

References