Coding

Part:BBa_K4242000

Designed by: zhelin Jiang   Group: iGEM22_CUG-China   (2022-07-12)
Revision as of 11:17, 28 September 2022 by Jiangzhelin (Talk | contribs)


FleQ

coding region for FleQ protein, which is a transcription factor that can be combine with FleN and c-di-GMP to regulate the gene transcription under the promoter Ppel.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 868
    Illegal XhoI site found at 1321
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 261
    Illegal NgoMIV site found at 1269
    Illegal NgoMIV site found at 1281
    Illegal NgoMIV site found at 1357
    Illegal NgoMIV site found at 1442
    Illegal AgeI site found at 670
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 283
    Illegal SapI.rc site found at 1216
    Illegal SapI.rc site found at 1225

Functional Parameters: CUG_China

Burden Imposed by this Part:

Model_of_pel_regulation_under_the_FleQ.jpeg
FleQ work mechanism
https://parts.igem.org/File:Model_of_pel_regulation_under_the_FleQ.jpeg
[edit]
Categories
Parameters
None