Composite

Part:BBa_K4229007:Design

Designed by: Nikita Edel   Group: iGEM22_Freiburg   (2022-09-28)
Revision as of 10:17, 28 September 2022 by NikitaEdel (Talk | contribs)

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RBS-TnaA-RBS-FRE


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 609
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 609
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 609
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 609
    Illegal AgeI site found at 1232
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1769
    Illegal SapI.rc site found at 1889


Design Notes

That's only half of the indigo pathway we provide.


Source

Made of BioBricks. BBa_K4229000, BBa_K4229001, BBa_K4229004 and BBa_K4229005. The whole sequence was synthesized.

References